A hands on educational module to teach aspects of human dietary health using fruit flies as a model
This module highlights an exercise to explore a variety of biological processes related to human nutrition and related health issues. The module capitalizes on the tremendous amount of knowledge on the life cycle, physiology, genetics, and behavior of Drosophila melanogaster to investigate the effects of diet on development, survival, behavior, environmental stressors, and physiological functions such as heart rate and neural-muscular function with sensory-motor integration. Students and teachers can develop their own flavor to the modules with exploration in the effect from various available mutations in Drosophila. Many stock lines are available which relate to human diseases providing the potential for students to pursue a wide-range of inquiries on human-related diseases such as diabetes and Metabolic Syndrome. This unit contains pedagogical support to assist teachers with facilitating open inquiry to acquire new knowledge. The interdisciplinary nature of the investigations provides opportunities for diverse connections to address authentic research questions related to diet and health in model invertebrate organisms. This module is presented in a variety of ways to be readily modified depending on equipment available in the classroom and the academic level of students. The modules were designed to be used in a hybrid or remote format with data provided or collected by in class participants.
Power point slides of presentation (ppt)
Egg - 1st instar - early 2nd instar - late 2nd instar - early 3rd - late 3rd - pupa
Resources: Papers and web links
1. Intro to A model for learning about aspects of metabolic syndrome (LaShay Byrd)
2. Diets for metabolic syndrome (LaShay Byrd and Jenni Ho)
3. Stages of larvae (Jenni Ho)
Collecting the staged larvae https://youtu.be/vJ7ZV0hxM5g
4. Making a simple Microscope (Ruth Sifuma)
5. Body wall movements (Brecken Overly)
6. Mouth hook movements (Hunter Maxwell & Crysta Meekins )
7. Ethograms and why ethograms- Brittany Slabach (still to add elk photos to movie)
8. Hat Assay (Maddie Stanback & Emma Rotkis)
9. Development of fruit flies-pupation rate (Clare Cole & Kay Johnson)
10. Anesthetize adult flies for moving them (Samantha Danyi)
11. Light and gravity sense in adults (Sushovan Dixit)
12. Effects of diet-ketogenic specific (Madan Subheeswar)
13. Measuring heart rate in larvae (Ann Cooper et al., 2009)
Cooper, A.S., Rymond, K.E., Ward, M.A., Bocook, E.L. and Cooper, R.L. (2009) Monitoring heart function in larval Drosophila melanogaster for physiological studies. Journal of Visualized Experiments (JoVE) 32: http://www.jove.com/video/1596/monitoring-heart-function-larval-drosophila-melanogaster-for )
14. The effect
of thermal stressors on larvae and adults fed various diets.
15. A summary of this educational module (LaShay Byrd).
16. Collecting eggs and staged larvae
17. A simple way to knock out flies to transfer a given number to another culture vial.
Sample larval behaviors:
Neat web link for middle and high school students and teachers about Fruit flies http://www.flyfacility.ls.manchester.ac.uk/forthepublic/
Potter, S., Krall, R.M., Mayo, S. Johnson, D., Zeidler-Watters, K., and
Cooper, R.L. (2016). Population dynamics based on resource availability
and founding effects: Live and computational models. The American Biology
Teacher 78(5): 396-403, ISSN 0002-7685, electronic ISSN 1938-4211 [PDF].
Participants designing this content are :
Brittany Slabach, Trinity University, One Trinity Place, San Antonio, TX 78212-7200
Tawny Aguayo-Williams, West Jessamine High School, 2101 Wilmore Rd, Nicholasville, KY 40356.
Byrd, L.S., Ho, A., Ho, J., Wilson, J., Rama, S., Veeraragavan, G., Middleton, D., Armstrong, K., Cole, C., Danyi, S., Johnson, K., Dixit, S., Subheeswar, M., Sifuma, R., Maxwell, H., Rotkis, E., Saelinger, C., Stanback, M., Overly, B., Stevens,K., Bender, A., Terry, L., Bowers, L., Newcomer, A., Butcher, B., Vance, A., Klein, A., McGraw, E., Cooper R.M., Noble, T., Ott, S., Robertson, R., Sweatt, C., Dixon, J., Biragane, J. and Cooper, R.L. - 675 Rose St., Department of Biology, University of Kentucky, Lexington, KY 40506-0225 USA.
maintained by Robin L. Cooper. Contact: RLCOOP1 at UKY.EDU