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Fall 2012:

Biology Building

Lecture: Biological Sciences, Rm. 107
LABS: See Black Board for course ( Lab web pages from 2011 have some resources you might find of use)

The information provided in the Fall 2012 course catalog regarding the location of the BIO 350 lab is not correct (for some sections). The Bio 350 teaching lab is located in the Multidisciplinary Sciences Building (MDS) in room 155B. This is the red brick and stucco building directly across the street from Biology (Thomas Hunt Morgan Building), right next to the UK clinic parking garage. All laboratory sections of the BIO 350 course meet in MDS 155B .

Lab
Section 001: 8-10:50 Monday (Ms. WenHui Wu wuwenhui2@gmail.com )
Section 002 :11-13:50 Monday (Mr. Josh Titlow joshtitlow@uky.edu)
Section 003: 14-16:50 Monday (Ms. WenHui Wu wuwenhui2@gmail.com )
Section 004: 8-10:50 Wednesday (Mr. Cliff Harpole ceharpole@uky.edu )
Section 005: 11-13:50 Wednesday (Mr. Cliff Harpole ceharpole@uky.edu
Section 006: 14-16:50 Wednesday (Mr. Josh Titlow joshtitlow@uky.edu )

Dr. Melody Danley
mlda227@uky.edu Office: Morgan building-216
Office Hours: email for time

Teaching assistants for the course:

Sec 001 & 003

Ms. WenHui Wu
wuwenhui2@gmail.com
Office: Room 226
Tel: 257-5950
Office Hours: email for appt.

Sec 004 & 005

Cliff Harpole
ceharpole@uky.edu
Biology Department

Sec 002 & 006

Josh Titlow
Biology Department
joshtitlow@uky.edu

Office: Room226
tel 257-5950

Course schedule (see below) (go to)

BIO350: Lecture Schedule for Bio 350, FALL 2012 (T & TH 8:00 AM- 9:15 AM)

Lecture, Day, Date, Topic Reading, Lecturer

1... Th... 8/23... Introduction to course and physiology...... Chapt 1&2, 3 (ECHO 360)

Stress relaxation here and here

To start thinking about energy watch this and enjoy . (here)

2...... T ...... 8/28 ...... Molecules, Energy and Biosynthesis ...... Chapt 3 (ECHO 360)
3...... Th...... 8/30 ...... Membranes, Channels and Transport/ Physical basis of neuronal function...... Chapt. 4, & 5 (Echo 360)
4 ...... T...... 9/4...... Physical basis of neuronal function ...... Chapt. 5 (Echo 360)
5 ...... Th...... 9/6...... Physical basis of neuronal function & start on Chapter 6...... Ch. 5/6 (Echo 360)
6 ...... T ...... 9/11...... Communication along and between neurons ...... Ch. 6 Chill out a bit with this here (Echo 360)

You can do it.... think of this mouse (here)

...... Th ...... 9/13 ...... Exam 1 ...... Ch1- 6part (PDF of test) (key of test) (#2, #37, #39 were miskeyed. They should be "B"- grade changes will occur)
7...... T ...... 9/18 ...... Communication along and between neurons...... Ch. 6 (Echo 360)
8 ...... Th ...... 9/20 ...... Communication along and between neurons ...... Ch. 6 (Echo 360)
9...... T ...... 9/25 ...... Sensory Mechanisms...... Ch. 7 (ECHO 360)
10 ...... Th ...... 9/27...... Sensory Mechanisms ...... Ch. 7 (ECHO 360)
11 ...... T ...... 10/2...... Sensory Mechanisms/ Organization of Nervous System ...... Ch. 7-8 (ECHO360)
12 ...... Th ...... 10/4...... Muscle ...... Ch. 10 (ECHO 360)
13 ...... T ...... 10/9...... Muscle & Behavior ...... Ch 10 (ECHO 360)
14 ...... Th ...... 10/11 ...... Muscle & Behavior...... Ch 10 (exam review.. ECHO360)

In case you need to chillout and relax from stress of starting back to school go here to hear
...... T ...... 10/16 ...... Exam 2 ...... Ch 6-10 (PDF of test, key is marked on test. Read note at top)
15 ...... Th...... 10/18 ......(UPDATED Behavior...... Ch 11 will be presented by Josh Titlow) (ECHO360)
16 ...... T ...... 10/23 ...... Endocrine ...Hypothalamus-Pituitary ...... Chapt. 9 (ECHO 360)
17 ...... Th...... 10/25 ...... Thyroid, parathyroid, adrenal and pancreas ...... Chapt. 9 (ECHO360)
18 ...... T ...... 10/30 ...... Wrap up endocrine ...... Chapt. 9 (ECHO360)
19 ...... Th 11/1 ...... Cardiovascular ...... Chapt. 9 (ECHO 360) Here is that crazy note I mentioned in class today about blood letting (here).
20...... T ...... 11/6 ...... Presidential Election Day (Academic Holiday)

21 ...... Th ...... 11/8 ...... Cardiovascular / Respiration ...... Chapt. 12 (ECHO 360) If you are interested in heart development ppt

To relax this weekend you need to hear this music maybe some more as well here

...... T ...... 11/13 ...... Exam 3 ...... 11, 9, 12 (answer key MS word file, scantron key PDF- # 41 was a miskey.. Answer should be C. )
22 ...... Th ...... 11/15 ...... Respiration ...... Chapt. 13 (ECHO360)
23 ...... T ...... 11/20 ...... Respiration ...... Chapt. 13 (ECHO360)
...... Th ...... 11/22 ...... Holiday - Thanksgiving
24...... T ...... 11/27 ...... Respiration / Ionic and Osmotic Balance......Chapt. 13 &14 (ECHO360)
25 ......Th ......11/29 ......Ionic and Osmotic Balance ......Chapt. 14 (ECHO360)
26 ......T ......12/4 ......Ionic and Osmotic Balance/ Digestion ......Chapt. 14 &15 (ECHO360)
27 ......Th...... 12/6...... Digestion / Heat and adaptations ......Chapt. 15-17 (ECHO360) (current article on poop) (also drugs & the GUT)

Chill out a bit with this here  & have a good break here

......W ......12/12 ......Wednesday......12/12/12; ......FINAL EXAM @ 8:00 AM room 107

BIO 350 Lab Schedule: See your black board page.

The outline presented above is a tentative plan for the semester. Specific topics and assignments are subject to change.

Principal Learning Objectives:
(Same learning objectives from http://courses.cit.cornell.edu/biog1440/)
1. To gain understanding of the principles of how animals work at the molecular, cellular, and systems levels. This understanding will come not by memorizing a large body of facts, but by developing mental models of the various physiological processes (e.g. transport processes, cell-cell signaling, metabolism, thermoregulation, circulation, respiration…) that underlie life.
2. To gain skill in thinking like a physiologist. This requires both asking and answering questions about physiology, and so involves observing phenomena, generating hypotheses about the underlying mechanisms, and devising tests of your hypotheses.
3. To appreciate the physiological similarities and differences between the physiologies of humans and other organisms, and so to understand how the study of physiology is thoroughly relevant to your own life and to the world around you.

Additional Lab Guidelines
An essential component of learning in physiology requires the use of live animals. It is impossible to demonstrate the full extent of possible responses through textbooks readings or lectures. As emerging professionals, it is expected that all students will demonstrate respect and maturity when working with these animals. If any disrespect or intentional cruelty is inflicted upon the animals, it may be reason to be expelled from the course with an "I" (incomplete), "W" (withdrawal), or automatic "E" (failing grade) depending the timing and degree of the offense.
No horse play, cutting up, playing around, etc. is allowed in the laboratory. There are many students coming and going in the lab throughout the day and materials are sometimes shuffled around. Squirting someone with a solution in a syringe or a bottle can be dangerous. You might "know" it is water but another person does not. A 3M KCl solution can easily be mistaken for water, and can be very harmful if squirted by accident in someone's eye.
We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Danley) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.
Every student will have to have completed the on line safety test and bring to the lab on the 1st day of your section meeting time. It is an easy test and you can take it multiple times until you get a 100 %. Either save and email your TA, or print it out and bring it to the first lab of the semester. The TA will check you off for having completed the exercise. The website for the safety test is:
http://ehs.uky.edu/classes/chemhyg/chemclass.php

Animal care:
Even though you will be using primarily invertebrate animals in these laboratories no torture of the animals is allowed. If any torturing occurs to the animals it is reason to be expelled from the course with an "I" (incomplete) or possible a "W" (withdrawal) depending the timing within the semester.
No horse play, cutting up, playing ball etc.. allowed in the laboratory period. Squirting someone with a solution in a syringe or a water bottle can be dangerous. You might know it is water but another person does not. 3M KCl can be very harmful if squirted by accident in someone's eye.
We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Cooper) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.

BIO 350 Laboratory Schedule

Mon Lab ......Wed Lab......Lab # :Topic
......8/27...... 8/29 ......1 Human: Heart and EKG modeling (report due Wed 9/5)
......9/3 .........9/5 ......No labs this week
......9/10 ......9/12 ......2 Crayfish: Electrical conduction of ventral nerve cord (report due Wed 9/19)
......9/17 ......9/19...... 3 Crayfish: Sensory integration (report due Wed 9/26)
......9/24 ......9/26 ......4 Crayfish: Intracellular physiology and ion movement (report due Wed 10/3)
......10/1 ......10/3 ......5 Crayfish: Synaptic transmission (report due Wed 10/10)
......10/8 ......10/10...... 6 Crayfish/Human: Muscle Contractions (report due Mon 10/15)
......10/15 ......10/17 ......7 Crayfish: Endcrn. Control of Hemolymph Glucose (report due Wed 10/24)
......10/22 ......10/24...... 8 Shrimp & Fruit fly: Heart rate control (report due Mon 10/29)
......10/29 ......10/31 ......9 Human: Respiration (report due Wed 11/7)
......11/5 ......11/7...... 10 Crayfish: Oxygen Consumption and Metabolism (report due Wed 11/14)
......11/12 ......11/14 ......11 Human: Salt and Water Balance (report due Mon 11/19)
......11/19 ......11/21 ......No labs this week: Thanksgiving
------------11/20----------- Final Draft of Journal Manuscript/Independent Research Project Due
......11/26 ......11/28 ......12 Crayfish: GI mobility (LAST LAB) (report due Mon 12/3)
......12/3 ......12/5 ......No labs this week.

IMPORTANT ACADEMIC DATES (Fall 2012)
Aug 22 First day of classes
Aug 28 Last day to add a class
Sept 3 Labor Day: Academic holiday
Nov 6 Election Day (Presidential): Academic Holiday
Nov 21-24 Thanksgiving: Academic holidays
Dec 7 Last day of classes
Dec 10-14 Final Exams

The outline presented above is a tentative plan for the semester.

Specific topics and assignments are subject to change.

Ch. 1: Chapter 1: 1, 2

........PDF of text handed out in class

................PDF 1-Evol. Med PDF or MS WORD

................PDF 2- Ice Fish PDF or MS WORD

Look at oxidative stress paper (PDF)

Ch. 2: NA

Ch. 3: 8, 12, 13, 18, 19, 20, 21, 30

Lactate, not pyruvate, is neuronal aerobic glycolysis end product: an in vitro electrophysiological study. {PDF}

Ch. 4: 1, 10,11, 13, 15, 19

Ch. 5: 3, 4, 6. 9, 16, 19

Chapter 6: 2, 3 . Also fun sensory activities in lab as well as going over the exam.

Chapter 6: 7, 8, 12, 14, 18

Chapter 7:1, 2, 3, 7, 10, 14

Mean quantal content HW Handed out. See these if you loose the one in recitation: PDF , MS word or Gif file

Chapter 8: 3, 6, 9

Week of Oct 5 discuss [PDF4] & [PDF5]

Chapter 10: 1, 3, 6, 12, 19, 22

READ OVER : PDF 1, PDF 2

here is a general link (go to)

Chapter 11: 2, 13

You should be able to answer these questions (you might see them again) !

Reading: in chapter 9

Questions for recitation

1. Discuss the differences between autocine, paracrine, neurocrine and endocrine secretion.

2. What are pheromones?

3. Explain how catecholamines can have so many different actions.

4. Discuss examples that illustrate the intimate functional association of the nervous and endocrine systems.

5. How can a single second messenger, (e.g. cAMP or IP3) induced by binding of different hormones, mediate different cellular responses in different tissues?

6. Explain how a small number of hormone molecules can elicit cell responses involving millions of times as many molecules?

To the issue about CHOLESTEROL and fluidity of biological membranes within normal temperatures. There maybe differences in the teaching on this point in Cell Biology and Animal Physiology so keep in mind which system, animal or bacteria, and the normal operating temperatures for the organisms.

In relation to Bio350 ANIMAL physiology and NOT bacterial cells the point is that cholesterol decreases fluidity in biological membranes of mammals.

Lets see the text by Lodish et al., (4th edition, 2000) Molecular Cell Biology

Page 165:

"Membrane cholesterol is another major determinate of bilayer fluidity....... The net effect of cholesterol on membrane fluidity varies, depending on the lipid composition. ...... At the high concentrations found in eukaryotic plasma membranes, cholesterol tends to make the membranes less fluid at growth temperatures near 37C."

Chapter 1-3 {ppt} {PDF}

Chapter 4 {ppt} {PDF}

Chapter 5 {ppt} {PDF}

Chapter 6 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 7 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 8 {ppt} {PDF}

Chapter 10 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 11 (ppt) {PDF}

chapter 9 {ppt-a; ppt--b} {PDF-a; PDF-b}

Clinical endocrine (ppt) overhead used in class Jpeg

chapter 12 {ppt-a; ppt-b} {PDF-a; PDF-b}

Clinical heart stuff (ppt) ionic current in pacing PDF

chapter 13 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 14 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 15 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 16 {ppt} {PDF}

chapter 17 {ppt} {PDF}

Neat Information:

Evolutionary Physiology 1994 (PDF)

About Myelin (PDF1) & (PDF2)

About folic acid (pdf)

Male Brain (pdf)

Drug MDMA (ectasy) (PDF)

neg pH (pdf)

Imprint genes (pdf)

Music & Brain (pdf)

Kiss & Run (pdf)

Neat PDF on nerve gas agents (PDF)

The leech model and glia cells (PDF)

OVERHEADS used in class: