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BIOLOGY 350- ANIMAL PHYSIOLOGY

LAB PAGE

Fall 2011

Lab, Room 202
Section 001: 8-10:50 Monday (Mr. Cliff Harpole ceharpole@uky.edu )
Section 002 :11-13:50 Monday (Ms. Rachel Holsinger, rcholsinger@uky.edu )
Section 003: 14-16:50 Monday (Ms. WenHui Wu wuwenhui2@gmail.com )
Section 004: 8-10:50 Wednesday (Mr. Cliff Harpole ceharpole@uky.edu )
Section 005: 11-13:50 Wednesday (Ms. Rachel Holsinger, rcholsinger@uky.edu)
Section 006: 14-16:50 Wednesday (Ms. WenHui Wu, wuwenhui2@gmail.com )



BIOLOGY 350- ANIMAL PHYSIOLOGY
Teaching assistants for the course:

WenHui Wu
wuwenhui2@gmail.com
Office: Room 226
Tel: 257-5950
Office Hours: email for appt

Sec 003 & 006

Rachel Holsinger
Biology Department
rcholsinger@uky.edu

Office: Room226
tel 257-5950

Sec 002 & 005

Cliff Harpole
ceharpole@uky.edu
Biology Department
Office: Room 302
tel: 257-2289

Sec 001& 004


LAB OUTLINE for Biology 350

BIO350: Lab Schedule for Bio 350, FALL 2011

Topic of the week:

BIO 350 Lab Schedule:

Mon Lab ......... Wed Lab ......... Lab # ......... Topic

Mon Lab ......... Wed Lab ......... Lab # ......... Topic
8/29 .................. 8/31.................. 1 ..................Modeling biological membranes (LAB PAGE-Model)
--- .......................9/7 ..................2 ..................Human: heart and EKG modeling ( LAB PAGE-EKG)

................(no Monday lab, all other labs meet. Monday sections will do this lab during the Thanksgiving week )

9/12 ..................9/14 ..................3 ..................Crayfish: Electrical conduction of ventral nerve cord (LAB PAGE- VNC)
9/19
..................9/21 ..................4 ..................Crayfish: Proprioception and MRO organ (LAB PAGE-MRO)
9/26
..................9/28.................. 5..................Crayfish: Sensory integration (LAB PAGE-6th Abd ganglion)
10/3
..................10/5 ..................6 ..................Crayfish: Intracellular physiology and ion movement (LAB PAGE- RP)
10/10
...............10/12.................. 7..................Crayfish and Rabbit: Neuromuscular physiology (LAB PAGE- muscle)
10/17
...............10/19 .................. 8 ................. Scientific writing, data formatting, and manuscript submission procedures
10/24
.............. 10/26................. 9 .................Invertebrates: Endocrine and behavior (LAB PAGE- Endocrine)
10/31
.............. 11/2 ................. 10 .................Human: Whole body physiology, effects of temperature and activity (LAB PAGE- Temp)
11/7
................. 11/9 ................. 11.................Shrimp & Fruit fly: Heart rate control (LAB PAGE-Shrimp heart)
11/14
.............. 11/16 ............... 12 .................Human & Crayfish: Respiration (LAB PAGE- Human breathing)
11/21
............... --- ...................... 2................. Human: Heart and EKG modeling ( LAB PAGE-EKG)

..............(no Wed lab. Monday lab meets)
------------11/22----------- Final Draft of Journal Manuscript Due
11/28
.............. 11/30 ................. 13 ............... Human & Crayfish:  Salt and water balance (renal physiology) (LAB PAGE- OSM )
12/5
................. 12/7 ................. 14................. Crayfish: GI mobility (LAB PAGE- GI)


LAB CONTENT:

Each lab exercise will have a www page associated with it. On the www page one will find movies, protocols to conduct the laboratory exercise, and resources to download. It is expected that one will view the movie and laboratory protocols prior to coming to the lab period.

Lab report guidelines: Download HERE      

The paper grading rubric  HERE

The write up for a lab exercises will need to be as if you were planning to submit it to a journal for peer review by other research scientist. The format will be as that described for the Journal of Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. Go to the www and look up information for authors on this journal's home page.

http://www.elsevier.com/wps/find/journaldescription.cws_home/525464/description#description

then go to guide to authors

http://www.elsevier.com/wps/find/journaldescription.cws_home/525464/authorinstructions

Then go to how to list your references and see format for references. Also you can open up a past article and see how they formated them.

Sample cover letter to paper-1; Sample paper-1; when published-PDF-1

Sample cover letter to paper-2; Sample paper-2; when published-PDF-2

More detail on the grading for the lab write up. PDF.

STATS, graphs and group data for the lab write up. PDF.

 

The results of your laboratory exercises and of all the other students across all sections will be posted on line. This data is to be used in your formal write ups. Put your results in perspective of the whole. Statistical analysis is REQUIRED and discussion of the analysis is expected.

Each laboratory section has 1 TA (graduate teaching assistant). All the sections have a www page with their data posted. This is the data that will be used in the formal write ups. The data will be discussed in lecture. The underlying concepts of the laboratory exercises will be on lecture exams. Content presented in lecture and laboratories are expected to be integrative such that content on quizzes in lecture or the lab can be mixed. No laboratory or section will have an advantage over the others as quizzes are different for each section and lecture exams are constructed so as not to cover content that particular sections might not have experience yet during the week.

The "manual" for the exercises is what will be posted on line within the www pages for each experiment. This is so the students will not have to buy a manual. You will be responsible for printing it out or having, in some means, the protocol to use for the laboratory period. Hard copies will not be provided. Overall this should save the students money. We cannot guarantee that the laboratories will have internet accessible during the lab period, but most likely one should be able to get on the www. So don't rely on the live feed from the www for your laboratory period. If you want, download on to a flash drive and bring to the lab.

The TA's will be grading quizzes and all material associated with the laboratories as well as being responsible for taking attendance checks.

The TA's are present to make the laboratory experience a valuable part of your learning experience.

Laboratory conduct:
Every student will have to have completed the on line safety test and bring to the lab on the 1st day of your section meeting time. It is an easy test and you can take it multiple times until you get a 100 % . Either save and email your TA or print it out and bring to the lab. The TA will check you off for having completed the exercise.
http://ehs.uky.edu/classes/chemhyg/chemclass.php
..............Drop down boxes Fill in: Thomas Hunt Morgan building, Room 202, phone 257- 5950

Animal care:
Even though you will be using primarily invertebrate animals in these laboratories no torture of the animals is allowed. If any torturing occurs to the animals it is reason to be expelled from the course with an "I" (incomplete) or possible a "W" (withdrawal) depending the timing within the semester.


No horse play, cutting up, playing ball etc.. allowed in the laboratory period. Squirting someone with a solution in a syringe or a water bottle can be dangerous. You might know it is water but another person does not. 3M KCl can be very harmful if squirted by accident in someone's eye.

We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Cooper) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.