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Fall 2015:

Biology Building

see this clip to get you thinking about course content:

https://www.youtube.com/watch?v=WVvKnq5XT-g

Lecture: Biological Sciences, Rm. 116 (ROOM CHANGE MDS 223)
LABS: See Black Board for course ( Lab web pages from 2011 have some resources you might find of use)

The Bio 350 teaching lab is located in the Multidisciplinary Sciences Building (MDS) in room 155B. This is the red brick and stucco building directly across the street from Biology (Thomas Hunt Morgan Building), right next to the UK clinic parking garage. All laboratory sections of the BIO 350 course meet in MDS 155B .

TA's:Adrienne Herrenbruck, Cole Malloy, Nadera Dabbain

Labs:
Section 001: 9:00-11:50 Monday
Section 002: 12:00-14:50 Monday
Section 003: 15:00-17:50 Monday
Section 006: 9:00-11:50 Wednesday (CLOSED OUT)
Section 004: 12:00-14:50 Wednesday
Section 005: 15:00-17:50 Wednesday

Dr. Melody Danley
mlda227@uky.edu

Office: MDS 125

Office Hours: email for time

Teaching assistants for the course:

Cole Malloy

camall2@uky.edu

Sec 001 & 006

Nadera Dabbain

Nida222@g.uky.edu

Sec 002 & 003

Adrienne Herrenbruck

adrienne.herrenbruck@uky.edu

Sec 004 & 005

Course outline PDF, MS word

(revised) for lab point corrections

PDF and MS WORD

Course schedule (see below) (go to)

BIO350: Lecture Schedule for Bio 350, FALL 2015 (T & TH 9:30 AM- 10:45 AM)

Lecture, Day, Date, Topic Reading, Lecturer

1... Th... 8/27... Introduction to course and physiology...... Chapt 1& 2, 3 (why comparative physiology-PDF) (podcast, ECHO360)

Stress relaxation here and here

....NOTE.............Lab this coming week (MS word, PDF) and a web page related to optogenetics (WWW). Power point used (PPT)

2...... T ...... 9/1...... Molecules, Energy and Biosynthesis ...... Chapt 3 (podcast A, B, & C, echo-360)

....... about COPD and O2 therapy (checkout this blog) and here is an Article (PDF)

LAB DATA sec 001 (PDF) sec 002 (PDF) sec 003 (PDF) sec 004 (PDF) sec 005 (PDF)

3...... Th...... 9/3...... Membranes, Channels and Transport/ Physical basis of neuronal function...... Chapt. 4, & 5 (podcast A & B, echo360)
4 ...... T...... 9/8...... Physical basis of neuronal function ...... Chapt. 5 (podcast, ..... echo 360...... Photo of over head notes)
5 ...... Th...... 9/10....... Physical basis of neuronal function & start on Chapter 6...... Ch. 5/6 (Podcast, echo 360)
6 ...... T...... 9/15....... Communication along and between neurons ...... Ch. 6 Chill out a bit with this here
(podcast and ECHO 360)

You can do it.... think of this mouse (here)

...... Th ......9/17...... Exam 1 ...... Ch1- 6 parts (follow up on Exam Essay questions... audio file)

7...... T ...... 9/22....... Communication along and between neurons...... Ch. 6 (podcast and echo 360)
8 ...... Th .....9/24...... Communication along and between neurons ...... Ch. 6 (podcast and echo360)
9...... T ...... 9/29....... Sensory Mechanisms...... Ch. 7 (podcast part 1, part 2, ECHO 360 ). (lecture assignment PDF)
10 ...... Th ......10/1....... Sensory Mechanisms ...... Ch. 7 ( podcast and ECHO 360)
11 ...... T ...... 10/6...... Sensory Mechanisms/ Organization of Nervous System ...... Ch. 7-8 (podcast and echo360).
12 ...... Th ...... 10/8....... Muscle ...... Ch. 10 (podcast and Echo 360) muscle man Youtube Muscle man EMG music

13 ...... T ......10/13....... Muscle & Behavior ...... Ch 10 (podcast and Echo360)
14 ...... Th ......10/15....... Muscle & Behavior...... Ch 10 & maybe Ch 11 (exam review)(podcast and Echo360)

In case you need to chillout and relax from stress go here

15 ...... T...... 10/20....... On line lecture... Behavior...... cont. Ch 11 (Oct. 23 at midnight your lecture assignment is due on safe assign through your black board)(podcast-1, podcast-2 and Echo360) (muscle topic ...ppt)

...... Th ...... 10/22....... Exam 2 ...... Ch 6-10 and maybe Ch 11 (except Ch 9)

16 ...... T ......10/27....... Endocrine ...Hypothalamus-Pituitary ...... Chapt. 9 (podcast and Echo360)
17 ...... Th...... 10/29..... Thyroid, parathyroid, adrenal and pancreas ...... Chapt. 9 (podcast and Echo360)
18 ...... T ......11/3...... Wrap up endocrine ...... Chapt. 9 (podcast part A, Part B and Echo360)
19 ...... Th... 11/5...... Cardiovascular ...... Chapt. 12 (podcast and Echo360) old paper on EKG and mean electrical axis (PDF)
20...... T ...... 11/10....... Cardiovascular ...... Chapt. 12 (podcast and Echo360)

21 ...... Th ...... 11/12...... Cardiovascular / Respiration ...... Chapt. 12 & 13. If you are interested in heart development ppt (podcast and no Echo360 but here is the ppt as a PDF).

Also a talk by Dr. Julie Pendergast will ocurr on this day. Her web page is here

To relax this weekend you need to hear this music maybe some more as well here

...... T ...... 11/17...... Exam 3 ...... 11, 9, 12, 13
22 ...... Th ...... 11/19.......Respiration ...... Chapt. 13(podcast and Echo360-messed up and it did not save)
23 ...... T ...... 11/24..... Respiration ...... Chapt. 13 (podcast and Echo360)

A very cool YouTube clip sent by your last year classmate: It is 4 minutes long and definitely a good watch. https://www.youtube.com/watch?v=YDspP4BhlTw

...... Th ...... 11/26....... Holiday - Thanksgiving
24...... T ...... 12/1....... Respiration / Ionic and Osmotic Balance......Chapt. 13 &14 (podcast and Echo360)
25 ......Th ......12/3.......Ionic and Osmotic Balance ......Chapt. 14 (podcast-did not work and Echo360)

http://www.ekgusa.com/ceol/abgmap/bgmodul/cebgquiz/bgmapqc.htm

Ion                         Normal blood concentration range (mmol.L−1)
Sodium                 135–145
Calcium                2.1–2.8
Potassium           ~3.5–5.0
Chloride               95–105
pH                          7.35-7.45
pCO2                     35-45
HCO3-                   22 to 26 mEq/liter ( some other sources 21–28 mEq/L)

26 ......T ......12/8.......Ionic and Osmotic Balance/ Digestion ......Chapt. 14 &15 (podcast and Echo360)

................GOOD TO LOOK OVER http://www.ekgusa.com/ceol/abgmap/bgmodul/cebgquiz/bgmapqc.htm

27 ......Th...... 12/10....... Digestion / Heat and adaptations ......Chapt. 15-17 ( gut brain connections) (current article on poop) (also drugs & the GUT) (podcast and Echo360) (Last day ppt and PDF)

Chill out a bit with this here  & have a good break here

A litte more chill'n here

...................................................Important link for relaxation https://www.youtube.com/watch?v=xfMWJi4req4

.........Monday 12/14/15.......FINAL EXAM @ 10:30 A.M-12:30 PM room 116 (150 points) (ROOM CHANGE to MDS 223)

(http://www.uky.edu/registrar/content/fall-final-exam-schedule)

BIO 350 Lab Schedule: See your black board page.

The outline presented above is a tentative plan for the semester. Specific topics and assignments are subject to change.

Principal Learning Objectives:
(Same learning objectives from http://courses.cit.cornell.edu/biog1440/)
1. To gain understanding of the principles of how animals work at the molecular, cellular, and systems levels. This understanding will come not by memorizing a large body of facts, but by developing mental models of the various physiological processes (e.g. transport processes, cell-cell signaling, metabolism, thermoregulation, circulation, respiration…) that underlie life.
2. To gain skill in thinking like a physiologist. This requires both asking and answering questions about physiology, and so involves observing phenomena, generating hypotheses about the underlying mechanisms, and devising tests of your hypotheses.
3. To appreciate the physiological similarities and differences between the physiologies of humans and other organisms, and so to understand how the study of physiology is thoroughly relevant to your own life and to the world around you.

Additional Lab Guidelines
An essential component of learning in physiology requires the use of live animals. It is impossible to demonstrate the full extent of possible responses through textbooks readings or lectures. As emerging professionals, it is expected that all students will demonstrate respect and maturity when working with these animals. If any disrespect or intentional cruelty is inflicted upon the animals, it may be reason to be expelled from the course with an "I" (incomplete), "W" (withdrawal), or automatic "E" (failing grade) depending the timing and degree of the offense.
No horse play, cutting up, playing around, etc. is allowed in the laboratory. There are many students coming and going in the lab throughout the day and materials are sometimes shuffled around. Squirting someone with a solution in a syringe or a bottle can be dangerous. You might "know" it is water but another person does not. A 3M KCl solution can easily be mistaken for water, and can be very harmful if squirted by accident in someone's eye.
We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Danley) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.
Every student will have to have completed the on line safety test and bring to the lab on the 1st day of your section meeting time. It is an easy test and you can take it multiple times until you get a 100 %. Either save and email your TA, or print it out and bring it to the first lab of the semester. The TA will check you off for having completed the exercise. The website for the safety test is:
http://ehs.uky.edu/classes/chemhyg/chemclass.php

Animal care:
Even though you will be using primarily invertebrate animals in these laboratories no torture of the animals is allowed. If any torturing occurs to the animals it is reason to be expelled from the course with an "I" (incomplete) or possible a "W" (withdrawal) depending the timing within the semester.
No horse play, cutting up, playing ball etc.. allowed in the laboratory period. Squirting someone with a solution in a syringe or a water bottle can be dangerous. You might know it is water but another person does not. 3M KCl can be very harmful if squirted by accident in someone's eye.
We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Cooper) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.

BIO 350 Laboratory Schedule

see lab web page

LABwww350FALL2015.htm

IMPORTANT ACADEMIC DATES (Fall 2015)

http://www.uky.edu/registrar/content/fall-2015-semester

Ch. 1: Chapter 1: 1, 2

........PDF of text handed out in class

................PDF 1-Evol. Med PDF or MS WORD

................PDF 2- Ice Fish PDF or MS WORD

Look at oxidative stress paper (PDF)

Ch. 2: NA

Ch. 3: 8, 12, 13, 18, 19, 20, 21, 30

Lactate, not pyruvate, is neuronal aerobic glycolysis end product: an in vitro electrophysiological study. {PDF}

Ch. 4: 1, 10,11, 13, 15, 19

Ch. 5: 3, 4, 6. 9, 16, 19

Chapter 6: 2, 3 . Also fun sensory activities in lab as well as going over the exam.

Chapter 6: 7, 8, 12, 14, 18

Chapter 7:1, 2, 3, 7, 10, 14

Mean quantal content HW Handed out. See these if you loose the one in recitation: PDF , MS word or Gif file

Chapter 8: 3, 6, 9

Week of Oct 5 discuss [PDF4] & [PDF5]

Chapter 10: 1, 3, 6, 12, 19, 22

READ OVER : PDF 1, PDF 2

here is a general link (go to)

Chapter 11: 2, 13

You should be able to answer these questions (you might see them again) !

Reading: in chapter 9

Questions for recitation

1. Discuss the differences between autocine, paracrine, neurocrine and endocrine secretion.

2. What are pheromones?

3. Explain how catecholamines can have so many different actions.

4. Discuss examples that illustrate the intimate functional association of the nervous and endocrine systems.

5. How can a single second messenger, (e.g. cAMP or IP3) induced by binding of different hormones, mediate different cellular responses in different tissues?

6. Explain how a small number of hormone molecules can elicit cell responses involving millions of times as many molecules?

To the issue about CHOLESTEROL and fluidity of biological membranes within normal temperatures. There maybe differences in the teaching on this point in Cell Biology and Animal Physiology so keep in mind which system, animal or bacteria, and the normal operating temperatures for the organisms.

In relation to Bio350 ANIMAL physiology and NOT bacterial cells the point is that cholesterol decreases fluidity in biological membranes of mammals.

Lets see the text by Lodish et al., (4th edition, 2000) Molecular Cell Biology

Page 165:

"Membrane cholesterol is another major determinate of bilayer fluidity....... The net effect of cholesterol on membrane fluidity varies, depending on the lipid composition. ...... At the high concentrations found in eukaryotic plasma membranes, cholesterol tends to make the membranes less fluid at growth temperatures near 37C."

Chapter 1-3 {ppt} {PDF}

Chapter 4 {ppt} {PDF}

Chapter 5 {ppt} {PDF}

Chapter 6 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 7 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 8 {ppt} {PDF}

Chapter 10 {ppt-a} {PDF-a}, {ppt-b} {PDF-b}

Chapter 11 (ppt) {PDF}

chapter 9 {ppt-a; ppt--b} {PDF-a; PDF-b}

Clinical endocrine (ppt) overhead used in class Jpeg PDF

Just some more on Muscle ppt

chapter 12 {ppt-a; ppt-b} {PDF-a; PDF-b}

Clinical heart stuff (ppt) ionic current in pacing PDF

chapter 13 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 14 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 15 {ppt-a; ppt-b} {PDF-a; PDF-b}

chapter 16 {ppt} {PDF}

chapter 17 {ppt} {PDF}

Neat Information:

Evolutionary Physiology 1994 (PDF)

About Myelin (PDF1) & (PDF2)

About folic acid (pdf)

Male Brain (pdf)

Drug MDMA (ectasy) (PDF)

neg pH (pdf)

Imprint genes (pdf)

Music & Brain (pdf)

Kiss & Run (pdf)

Neat PDF on nerve gas agents (PDF)

The leech model and glia cells (PDF)

Muscle Growth (PDF)

Skeletal muscle (link to actin and myosin ratios....here)

Heart. MEA in rats PDF

OVERHEADS used in class: