BIO 510 2007

Second Half with Pete Mirabito

Lab Protocols

Day by Day Outline of the Second Half of BIO 510

Vector NTI Introduction and Homework Assignments

Experiment 1: Site-directed Mutagenesis

Experiment 2: Random Mutagenesis

Experiment 3: Transposon Mutagenesis

Experiment 4: Cre-lox Recombination and Protein Expression in E. coli

Experiment 5: Gene Knock-outs

pET15 in L21(DE3)pLysS: Induction Protocol

Western Blot Probing Protocol

User Manuals

Vector NTI User Manual

Strategene’s QuickChange Mutagenesis Kit

Strategene’s GeneMorph Mutagenesis Kit

NEB’s GPS Transposon Mutagenesis Kit

Original Univector Publication

Generating Gene Knockout Constructs Using Fusion PCR

Gene Knockouts Using a Ku70 Mutant Background

Grades

Homework Grades

Notebook Grades

Exam 2 Grades

Cumulative Course Grades and Final Letter Grade