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Project 1: Modulation alteration in transmission by extracellular K+ or by evoked stimulation frequency. See below Project 2: Synaptic transmission: Effects of intracellular pH on transmission Project 3: Assessment of behaviors (GO TO) FREE
NEUROPHYSIOLOGY TEXT BOOK ON LINE Presentation at SICB Jan 2015 ( movie Windows media and ppt ) Project
1 Information: (IRB) High school
student project (KY) - |
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The learning objective of this current activity is to be able observe and measure quantal synaptic vesicular release of neurotransmitter at the crayfish and Drosophila neuromuscular junctions (NMJ) as well as analyze electrical activity within the CNS of these animals. Students will participate in authentic scientific data gathering and help to improve the methods for this science project. The primary data is obtained in one lab and then posted on line for participants to download. The synaptic responses are experimentally recorded when a motor nerve is electrically stimulating for inducing evoked responses. Spontaneous vesicular events are also recorded in the absence of stimulation or between evoked stimulations. The participants estimate means quantal content with three different approaches direct counts, amplitude measurements, and charge (area) measurements. Other forms in analysis of activity from CNS circuits will also be tackled.
Parts for Project 1:
PPTs: Intro on synaptic transmission;.... Intro on Channel Rhodopsins ..... quantal variation ppt
Movies: Zana, Tori intro, Tori measures , This movie is for 2018 in only having to count quantal events on YouTube (https://youtu.be/XMg9v0RQLdQ) for the explanation or you can download 222 MB file here Windows Media by Robin
Download files
Data files posted for Project 1:
{1 mM Ca2+ in the bath}
File 1: 20, 40 and 60 Hz with 30 sec wait between each trail . Each condition repeated 10 times with 10 stimuli each..
01-17-2017 prep 1 -30 sec delays .............. DATA SHEET Excel file for 1st, 5th and 10th trials
A file not for analysis but to see the evoked responses EPSPs with only 10 sec between trails
EPSP views prep 2- 10 sec delays
File 2: This is now of the same type of experiments but instead of 1 mM Ca2+ in the bath we only have 0.1 mM Ca2+. The 1st part of the file shows the change from 1 mM to 0.1 mM so you can see the effect on the evoked release. Then we go to high gain to examine minis at 20 Hz, 40 Hz and 60 Hz stimulations of 10 pulses with 30 sec intervals for the 10 trials for each condition. The file 0'1mmCa 30 second interval 1-23-17.adicht is name as such since saving the file as 0.1 some times causes windows machines to go crazy with the " . "
File 3: This file is with 20 stimulations within a train. The frequencies are the same as for the 10 stimultions within a train (20 Hz, 40 Hz and 60 Hz) with 30 seconds between trains. Again lets only analyize the frequncy of minis after the 1st, 5th and 10th stimulation trains for each of the frequencies. The file is here And this is with 1.0 mM Ca2+ in the bath.
File 4: TBA
File 5: TBA
Files for Project 2:
Email for the files (RLCOOP1 at uky.edu)
Teams:
High Schools in Kentucky, USA
University of Kentucky, Lexington, KY, USA
Wright State University, Dayton, OH, USA
Seoul National University, South Korea
Korean Military Academy, South Korea
Univesity of Salahaddin, Erbil, Iraq
Universität Leipzig, Germany
University of Padova, Italy