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MIDDLE SCHOOL SCIENCE TEACHERS WORKSHOP

FCPS & UK

http://www.uky.edu/

 

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Summer 2005

Biology Build.

RM 215 (2nd floor lab)

Jump to Participants www page

Jump to Action Shots www page


Participants:

Jump to NEW www page as there are many class photos to see. (click here)


 
Instructors:

Mr. David Taylor....

Science Specialist Fayette County School Board Department of Student Achievement CIAS,
Potts Annex, 303 Fayette County PublicSchools
701 East Main St. Lexington, KY 40475

dtaylor@fayette.k12.ky.us

Dr. Robin L. Cooper

RLCOOP1@pop.uky.edu

Office: Morgan building-B08
Tel: 257-5950

WWW Home page (go to)


Dr. Kert Viele

(not the horse-the one on it)

Dept. of Statistics.

UK home page (go to)
viele@ms.uky.edu

Teaching assistants for the workshop:

Sameera Dasari

sdasa2@uky.edu

Nicolas Badre

READ ALL ABOUT HIM (click on link) http://www.uky.edu/PR/News/050426_beckman_scholars.htm nick_badre@hotmail.com

Justin Blackbun

jlblac3@uky.edu

High School Science teachers integrating with the Middle School teachers in the workshop:

Ms. Heidi Melcher Anderson
Paul Laurence Dunbar High School
1600 Man'O' War Boulevard
Email: HANDERSO@Fayette.k12.ky.us

www page: (go to)

 

Ms. Barrie Hart
Paul Laurence Dunbar High School
1600 Man'O' War Boulevard
Email: bhart@fayette.k12.ky.us

www page: (go to)


Power Point Files (go to)Workshop outline (PDF) Readings (go to)

General Movies (go to)

Barrie Hart's in class HR movie made with Taylor's microscope camera (go to) 60 MB file

Photos (go to new www page)

www page- for class action

Protocols and Procedures (go to)

OUTLINE

Summer 2005

Day 1 (Monday 25, 2005)
1. 9:00 AM Find Room 215 Biology Building
...................Overview of workshop- solve all minor details (housing). Overview of campus/Lexington
2. Laboratory safety- where to go and what to do. Take UK on-line lab safety and hazardous waste safety tests.
3. Use of laboratory equipment. David Taylor goes over LABPRO Instrumentation.
4. Animal care: Fruit flies (general biology- life history).
5. Course goals. How to keep a notebook.
6. Set flies up for later studies. 2 hour egg pulse. Let sit over night at room temp. (next day look at 1st instars)
7. Show movies for behavioral assays.
8. Lunch 12-1.
9. 1:00 PM Learn how to make measures for data collection.

BREAK into groups-

G1: Behavior of larvae
G2: Heart rate measures of larvae
G3: Adult behaviors

(2:00-4:00PM). Dr. Viele from the Dept of Statistics will talk on "How to design an experiment & use of statistics"
Go over graphing software implementation on lap top computers.

Day 2 (Tuesday, July 26)
AM: Examine effects of nicotine on behaviors - sensory functions.
PM: Analysis of data- graph and statistics
The instars set up on Monday are ready to prepare for addiction assay.

Day 3 (Wednesday, July 27)
AM: start on dose-response curves
PM: Analysis of data- graph and statistics
Class data sets compiled. See what is left to complete with behaviors.
The instars set up on Monday are ready to assay (addiction assay for early 3rd instars).

Day 4 (Thursday, July 28)
AM: How to implement what is learned into their classrooms?
Talk about the neural circuit and actions of Nicotine.
PM: HW assignment- write up experimental design for their classroom and list equipment needs. Start to read primary literature on animal behavior and pharmacology.

Day 5 (Friday, July 29)
AM: Discuss HW assignment and literature provided the day before.
PM: Develop detailed information content for Teachers' students in Middle school. Set up for experiments on Monday.

Day 6 (Monday, Aug. 1)
AM-PM: Complete experiments and data gathering. Dose response curves of pharmacological manipulations.

Day 7 (Tuesday, Aug. 2)
AM-PM: Complete experiments and data gathering. Dose response curves of pharmacological manipulations.

Day 8 (Wednesday, Aug. 3)
AM: Statistical computation with Dr. Viele (UK Dept. of Statistics). Ten work stations will be implemented. The goals here are to teach the basics to process data. Which statistical test to use for the data collected? How to describe the data and quantify statistically?
PM: Working on statistical analysis and completing does response experiments.
Teachers mix teams and a partner teacher the other one what experiments they did and go over their data as well as analysis.

Day 9 (Thursday, Aug 4)
AM: Compare wild type and mutant lines of Drosophila.
PM: Graph results, writing up, drafting manuscript. Prepare ppt for presentations.

Day 10 (Friday, Aug 5)
AM: Behavioral assays in Crayfish. Life history and setting up experiments for classroom use in Middle schools.
PM: Closing session- feed back from teachers. Go over where to obtain the goods they need for their classrooms.
Present some stand ppt files that can be used by the teachers for their classes. Burn CDs and distribute content folders.

PM: Each dissect fruit fly larvae & learn to measure behaviors
PM: Learn how to make measures and analysis on the computer of data collected the day before.
BREAK into 3 groups- G1: Behavior of larvae
G2: Heart rate measures of larvae
G3: Adult behaviors
Day 2 (Tuesday, July 26)
AM: Examine effects of neuromodulators on behaviors - sensory functions.
PM: Analysis of data- graph and statistics

Day 3 (Wednesday, July 27)
AM: start on dose-response curves
PM: Analysis of data- graph and statistics

Day 4 (Thursday, July 28)
AM: How to implement what is learned into their classrooms?
PM: HW assignment- write up experimental design for their classroom and list equipment needs. Start to read primary literature on animal behavior and pharmacology.

Day 5 (Friday, July 29)
AM: Discuss HW assignment and literature provided the day before.
PM: Develop detailed information content for Teachers' students in Middle school. Set up for experiments on Monday.

Day 6 (Monday, Aug. 1)
AM-PM: Complete experiments and data gathering. Dose response curves of pharmacological manipulations.

Day 7 (Tuesday, Aug. 2)
AM-PM: Complete experiments and data gathering. Dose response curves of pharmacological manipulations.

Day 8 (Wednesday, Aug. 3)
AM: Statistical computation with Dr. Viele (UK Dept. of Statistics). Ten work stations will be implemented. The goals here are to teach the basics to process data. Which statistical test to use for the data collected? How to describe the data and quantify statistically?
PM: Working on statistical analysis and completing does response experiments.
Teachers mix teams and a partner teacher the other one what experiments they did and go over their data as well as analysis.

Day 9 (Thursday, Aug 4)
AM: Compare wild type and mutant lines of Drosophila.
PM: Graph results, writing up, drafting manuscript. Prepare ppt for presentations.

Day 10 (Friday, Aug 5)
AM: Behavioral assays in Crayfish. Life history and setting up experiments for classroom use in Middle schools.
PM: Closing session- feed back from teachers. Go over where to obtain the goods they need for their classrooms.
Present some stand ppt files that can be used by the teachers for their classes. Burn CDs and distribute content folders.


Power point files

1. Kert Viele's statistical talk (ppt)

2. Nick Badre's examples of behaviors for flies (ppt)

3. Ms. Barrie Hart's HR movie (60MB download) (click here)


Readings

1. Paper on Nicotine (PDF)

2. Paper on use of invertebrates to study drugs of abuse (PDF)

3. Paper of fly behavior #1 (PDF)

4. Paper on nictoine and psychiatric disease (pdf)


Protocols and Procedures

1. Apple juice plates:

for 1/4 liter:

Agar 7.6 grams; distilled water 250 mL; table Sugar 8.33 gram; Apple juice 83 mL

Add the agar, water and sugar. Heat until bubbles appear (boiling). Use a magnetic stirrer and a magnet for chemicals in the solution when heating.

Be careful not to let the solution boil over. Then add the apple juice and swish the container by hand in mix in.

Pour the solution into the plates and let them sit for 15 minutes until they are hard. Cover and place in a plastic bag in the refrig.

The Agar is just plan "agar agar". All other compunds can be bought in local market.

 

2. FLY FOOD- see the following link for a full listing of different types of food.

http://flystocks.bio.indiana.edu/media-recipes.htm


3. Drosophila Stock Center at Indiana University -- order all types of mutant flies

- go here http://flystocks.bio.indiana.edu/

 

 

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