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SEE MRO LAB and crab joint receptor labs for overview movies and protocols

Published protocol (text and movie crab leg PD) : http://www.jove.com/video/51050/proprioception-tension-receptors-crab-limbs-student-laboratory

Published protocol (text and movie crayfish MRO) :
http://www.jove.com/video/2323/muscle-receptor-organs-crayfish-abdomen-student-laboratory-exercise

A start on the posters: (Sample from past years)

Effects of LPS endotoxin on firing frequency of proprioceptive neurons in crustaceans

Refs for the bacterial exposures in crayfish and sea crustaceans PDF 1 & PDF 2

Papers from past Bio446/650 classes

Dayaram,V., Malloy, C., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., and Cooper, R.L. (2017). Stretch activated channels in proprioceptive chordotonal organs of crab and crayfish are sensitive to Gd3+ but not amiloride, ruthenium red or low pH. IMPLUSE The Premier Undergraduate Neuroscience Journal. [PDF] https://impulse.appstate.edu/issues/2017

Malloy, C., Dayaram,V., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., Weineck, K. and Cooper, R.L. (2017). The effects of neighboring muscle injury on proprioception responses in crayfish and crab. J. of Exp. Zoology. 327(6):366–379. [PDF]

Dayaram,V., Malloy, C., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., and Cooper, R.L. (2017). The effect of CO2, intracellular pH and extracellular pH on mechanosensory proprioceptor responses in crayfish and crab. American Journal of Undergraduate Research 14(3):85-99 [PDF].

Wycoff, S., Weineck, K., Conlin, S., Grau, E., Bradley, A., Cantrell, D., Eversole, S., Grachen, C., Hall, K., Hawthorne, D., Kinmon, C., Ortiz Guerrero, P., Patel, B., Samuels, K., Suryadevara, C., Valdes, G., Ray, A., Fleckenstein, L., Piana, E., Cooper, R.L. (2018) Investigating potential effects of clove oil (eugenol) in model crustaceans. IMPLUSE pp. 1-21 https://impulse.appstate.edu/articles/2018/effects-clove-oil-eugenol-proprioceptive-neurons-heart-rate-and-behavior-model-crustac

Over all PDFs related to projects

Rydquistetal2007MRO-crayfish

PPT talk on PD organ

Molecular mechanisms 2011 of mechanotransduction

The sensation of stretch 2014

MRO-protocol MS word or PDF

Crab pd lab protocol MS word or PDF

Stretch activated review 2015

proprioception review 2012

review 2012 touch-Geffeney

Bewick and Banks 2015-Mammal muscle spindle

Biochemical Basis of Touch-molecular-review-2002

Signaling by Sensory Receptors

Mechanosensory molecules and circuits in C elegans-review

Pickpocket1 Is an Ionotropic Molecular Sensory Transducer

amiloride block in Drosophila neurons-2nd paper..... photo amiloride block in Drosophila MD neurons

Piezo Is Essential for Amiloride-Sensitive2015

Specfic to :

After movies:

Specfic to : Effects of LPS endotoxin on firing frequency of proprioceptive neurons in crustaceans

DATA SETS: Pseudomonas aeruginosa (P.a); Serratia marcescens (S.m.)

crab P.a., prep 2

crayfish P.a., prep 3 - differentiation between static and dynamic.

crayfish S.m., prep 1. Prep 5 is very noisy to show contrast.