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BIOLOGY 350- ANIMAL PHYSIOLOGY

LAB PAGE

Fall 2010

NOTE : about MONDAY LAB (PDF)


Back to LECTURE BIO350 page

 

STATEMENT FROM DEPARTMENT CHAIRPERSON

Science is not only a unique way of knowing the natural world, it is a very specific way of doing. It is an exercise in exploring the natural world by forming a testable hypothesis, designing an experiment, both physically and intellectually, and performing that experiment. At the end, it enables us to either confirm our suspicions as to how the world works or to adjust our hypotheses. The new Biology curriculum and the new core labs in the Biology department are designed to enable committed Biology students to not only gain that scientific "way of knowing" but to gain the tools for "doing" science. The Bio 350 laboratory is our first salvo into that world. Have fun with it, embrace it, and it will change the way you live!

Vincent M. Cassone, Ph.D.
Professor and Chair

Lecture: Biological Sciences, Rm. 107
LABS:

Sec 001 : Monday 9:00 - 11:50 AM
Sec 002 :
Monday 12:00 - 2:50 PM
Sec 003 :
Wednesday 8:00-10:50 AM
Sec 004 :
Wednesday 11:00 -1:50 PM
Sec 005 :
Wednesday 2:00 - 4:50 PM
Sec 006 :
Friday 9:00 - 11:50 AM


BIOLOGY 350- ANIMAL PHYSIOLOGY
Teaching assistants for the course:

WenHui Wu
wuwenhui2@gmail.com
Office: Room 226
Tel: 257-5950
Office Hours: email for appt

Sec 001 & 004

Tim Bradshaw

ttbrad2@email.uky.edu
Biology Department
Office: Room
tel 257-5950 (Cooper's lab)

Sec 002 & 006

Cliff Harpole
ceharpole@gmail.com
Biology Department
Office: Room 302
tel: 257-2289

Sec 003 & 005

Facilitators for the course:

Brittany Baierlein [brittany.baierlein@uky.edu]

Sec 006 : Friday 9:00 - 11:50 AM

Bonnie Leksrisawat
[bonnyjean13@gmail.com]

Sec 004 : Wednesday 11:00 -1:50 PM

Ashley Buchanan
[akbuch2@email.uky.edu]

Sec 001 : Monday 9:00 - 11:50 AM


Randi Barnett
[rrbarn4@uky.edu]

Sec 005 : Wednesday 2:00 - 4:50 PM

Sarah O'Nan
[seonan2@uky.edu]

Sec 003 : Wednesday 8:00-10:50 AM

AlisonThurow
[alison.thurow@uky.edu]

Sec 002 : Monday 12:00 - 2:50 PM


LAB OUTLINE for Biology 350

BIO350: Lab Schedule for Bio 350, FALL 2010

Topic of the week:

Week 1 (Aug 30) Modeling biological membranes and learn to use volt meters (LAB PAGE-Model)
Week 2 (Sept 6) Human heart EKG (LAB PAGE-EKG)
NOTE : about MONDAY LAB (PDF)

..............{Sept 6, (no Monday lab, all other labs meet. Monday sections will do this lab the following week )}

Week 3 (Sept 13 ) Electrical conduction properties (crayfish ventral nerve cord) (LAB PAGE- VNC) NOTE : about MONDAY LAB (PDF)
Week 4 (Sept 20) Proprioception. Crayfish MRO organ. (LAB PAGE-MRO)
Week 5 (Sept 27) Integration of sensory - crayfish 6th abd ganglion and primary neurons (LAB PAGE-6th Abd ganglion)
Week 6 (Oct 4) Heart rate in crustaceans/insects (Ghost shrimp/Drosophila lab) (LAB PAGE-Shrimp heart)
Week 7 (Oct 11) Intracellular recordings- ion movements (RP-ions, Nernst eq, GHK, crayfish abdomen deep extensor prep) (LAB PAGE- RP)
Week 8 (Oct 18) Synaptic physiology- crayfish abdomen deep extensor phasic and tonic muscles (LAB PAGE- muscle)
Week 9 (Oct 25) Respiration in humans. (LAB PAGE- Human breathing)
Week 10 (Nov 1) Endocrine and behavior: Invertebrates (LAB PAGE- Endocrine)
Week 11 (Nov 8) Water balance in invertebrates (osmotic stress in crayfish) (LAB PAGE- OSM crayfish)
Week 12 (Nov 15) GI recording in crayfish. Pharmacology (recordings of muscle contraction with force transducers- crayfish gut) (LAB PAGE- GI)

...........{Nov 22—NO LABS THIS WEEK for the Wed and Friday sections } NOTE : about MONDAY LAB (PDF)

Week 13 ( Nov 29) Temperature and activity. (Temperature and Physiological stress). (LAB PAGE- Temp)

Last week of classes… last of the formal write ups due.


LAB CONTENT:

Each lab exercise will have a www page associated with it. On the www page one will find movies, protocols to conduct the laboratory exercise, and resources to download. It is expected that one will view the movie and laboratory protocols prior to coming to the lab period. There will be a prequiz upon arriving in the lab. This quiz will also be used for purposes of an attendance check. The quiz will address the purpose of the laboratory exercise and the basics of how it will be conducted.
There will be two formal laboratory reports due. One will be due by midterm and the other will be due by the beginning of dead week (assignments are not suppose to be given during dead week by UK policy rules). The write up for two lab exercises will need to be as if you were planning to submit it to a journal for peer review by other research scientist. The format will be as that described for the Journal of Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. Go to the www and look up information for authors on this journal's home page.

SAMPLES: Letter to students via BB (MS word, PDF)

Sample cover letter to paper-1; Sample paper-1; when published-PDF-1

Sample cover letter to paper-2; Sample paper-2; when published-PDF-2

More detail on the grading for the lab write up. PDF.

STATS, graphs and group data for the lab write up. PDF.

 

The results of your laboratory exercises and of all the other students across all sections will be posted on line. This data is to be used in your formal write ups. Put your results in perspective of the whole. Statistical analysis is REQUIRED and discussion of the analysis is expected.

Each laboratory section has 1 TA (graduate teaching assistant) and 1 facilitator (an undergraduate student) assigned. The "facilitator" will be collecting data from your experiments weekly and posting it on a www page for your section. All the sections have a www page with their data posted. This is the data that will be used in the formal write ups. The data will be discussed in lecture. The underlying concepts of the laboratory exercises will be on lecture exams. Content presented in lecture and laboratories are expected to be integrative such that content on quizzes in lecture or the lab can be mixed. No laboratory or section will have an advantage over the others as quizzes are different for each section and lecture exams are constructed so as not to cover content that particular sections might not have experience yet during the week.

The "manual" for the exercises is what will be posted on line within the www pages for each experiment. This is so the students will not have to buy a manual. You will be responsible for printing it out or having, in some means, the protocol to use for the laboratory period. Hard copies will not be provided. Overall this should save the students money. We cannot guarantee that the laboratories will have internet accessible during the lab period, but most likely one should be able to get on the www. So don't rely on the live feed from the www for your laboratory period. If you want, download on to a flash drive and bring to the lab.

The TA's will be grading quizzes and all material associated with the laboratories as well as being responsible for taking attendance checks. The facilitators will be helping in conducting the experiments and gathering data from you. They will have your email address and you are responsible for getting information to them in a reasonable amount of time so the spread sheets can be posted on line.

The TA's and facilitators are present to make the laboratory experience a valuable part of your learning experience.

Laboratory conduct:
Every student will have to have completed the on line safety test and bring to the lab on the 1st day of your section meeting time. It is an easy test and you can take it multiple times until you get a 100 % . Either save and email your TA or print it out and bring to the lab. The TA will check you off for having completed the exercise.
http://ehs.uky.edu/classes/chemhyg/chemclass.php
..............Drop down boxes Fill in: Thomas Hunt Morgan building, Room 202, phone 257- 5950

Animal care:
Even though you will be using primarily invertebrate animals in these laboratories no torture of the animals is allowed. If any torturing occurs to the animals it is reason to be expelled from the course with an "I" (incomplete) or possible a "W" (withdrawal) depending the timing within the semester.


No horse play, cutting up, playing ball etc.. allowed in the laboratory period. Squirting someone with a solution in a syringe or a water bottle can be dangerous. You might know it is water but another person does not. 3M KCl can be very harmful if squirted by accident in someone's eye.

We will use a fixative in the lab. The fix solution is a Bouin's solution (Prepared with saturated picric acid, formaldehyde and acetic acid; Sigma-Aldrich Co.). Some people are very allergic to the vapors of formaldehyde. If you know you are allergic please inform the instructor (Dr. Cooper) as soon as possible so we can make alternative plans. The fix solution is to remain in the vented hood.