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Joint receptor experiments for presentations
& publications

The effect of CO2, intracellular and extracellular pH on mechanosensory proprioceptor responses in crayfish and crab.


An undergraduate education module based on a research question: The effects of neighboring muscle injury on proprioception responses in crayfish and crab.

Examining the pharmacology of stretch activated ion channels on mechanosensory proprioceptor responses in crayfish, crab and Drosophila.

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SEE MRO LAB and crab joint receptor labs for overview movies and protocols

Published protocol (text and movie crab leg PD) : http://www.jove.com/video/51050/proprioception-tension-receptors-crab-limbs-student-laboratory

Published protocol (text and movie crayfish MRO) :
http://www.jove.com/video/2323/muscle-receptor-organs-crayfish-abdomen-student-laboratory-exercise

 

 

A start on the three posters: CO2-pH ....... K+ & muscle juice....... Pharmacology of stretch activated

 

 

Over all PDFs related to projects

Rydquistetal2007MRO-crayfish

PPT talk on PD organ

Molecular mechanisms 2011 of mechanotransduction

The sensation of stretch 2014

MRO-protocol MS word or PDF

Crab pd lab protocol MS word or PDF

Stretch activated review 2015

proprioception review 2012

review 2012 touch-Geffeney

Bewick and Banks 2015-Mammal muscle spindle

Biochemical Basis of Touch-molecular-review-2002

Signaling by Sensory Receptors

Mechanosensory molecules and circuits in Celegans-review

Specfic to : CO2-pH

List of papers on pH and synaptic transmission-ms word

update presentation pHfor lab ppt

PowerPoint cell pH regulation-A

SFN-Warlen Presentation--ppt poster

sleep CO2-2014

phCO2 clam-2014

Gutknecht -1973 Science

Sinning Hubner Review 2013 pH

PhReview by Putman-2001

Stretch-activated channels- Kchannel activated by low pH inside

low pH -Leech inhibited at pH5

SIDS, pH, CO2.....2016 paper

 

Specfic to : Effects of neighboring muscle injury

membrane Physiology 1

Nobel prize Na-K pump

glia and K

jphysiol1966crayfish muscle-K

J Physiol-1960-Hodgkin-370-85

crayfish axon K stimulation

1967 Strickholm Wallin

There are two movies on YOUTUBE which walk an instructor and students through the main points of this lab exercise in relation to muscle injury.
Part 1: Resting membrane potentials with varied K+ and muscle juice.
https://www.youtube.com/watch?v=f7YySz7Rz9Q
Parts 2 & 3: Sensory stimulation and motor nerve recording; Synaptic responses at NMJ.
https://www.youtube.com/watch?v=mrRFVcrwJhA

 

Specfic to : pharmacology of stretch activated ion channels

Pickpocket1 Is an Ionotropic Molecular Sensory Transducer

amiloride block in Drosophila neurons-2nd paper..... photo amiloride block in Drosophila MD neurons

Piezo Is Essential for Amiloride-Sensitive2015

acid sensing block by amilioride

Suslak2015droPiezo80knockdownwithRred

nature10812-rutheniumRed-50µM

Piezo fly-KIM- 2012

piezo in stem cell sensory neurons-human

PickpocketSensory-Heart 2012

stretch activated channels heart Fly[1]

stretch activated channels heart Fly-2

DATA SETS:

Crab: PDcrab-prepA, prepB, prepC, prepD, prepE, prepF (CHART FILES)

Crayfish: